Characterization of two cryptic plasmids from Spiroplasma citri and occurrence of their DNA sequences among various spiroplasmas
Identifieur interne : 000290 ( France/Analysis ); précédent : 000289; suivant : 000291Characterization of two cryptic plasmids from Spiroplasma citri and occurrence of their DNA sequences among various spiroplasmas
Auteurs : C. Mouches [France] ; G. Barroso [France] ; A. Gadeau [France] ; J. M. Bové [France]Source :
- Annales de l'Institut Pasteur / Microbiologie [ 0769-2609 ] ; 1984.
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Abstract
Summary: Two plasmids, pMH1 with 7 kilobase pairs (Kbp) and pM41 with 8 Kbp, were purified from Spiroplasma citri strains MH and M4, respectively, and characterized by restriction mapping. Upon in vitro DNA recombination with plasmid pBR328 as a vector, pMH1 was cloned in Escherichia coli. Radioactive probes specific of the plasmids were used to investigate the occurrence of pMH1 and pM41 DNA sequences among various spiroplasmas. pM41 or a closely related plasmid was found in three other S. citri strains and also seems to be present as an 8-Kbp plasmid in three spiroplasmas not belonging to the S. citri species. Up to now, pMH1 had been found as a free 7-Kbp plasmid only in the S. citri strain MH. However, DNA sequences corresponding to the entire pMH1 DNA have been found to be integrated in the high-molecular-weight-DNA molecules and perhaps the chromosomal DNA itself of two other S. citri strains. DNA sequences hybridizable with pMH1 DNA have also been found to be integrated into high-molecular-weight-DNA molecules from several spiroplasma strains not belonging to the S. citri species.
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DOI: 10.1016/S0769-2609(84)80054-X
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Upon in vitro DNA recombination with plasmid pBR328 as a vector, pMH1 was cloned in Escherichia coli. Radioactive probes specific of the plasmids were used to investigate the occurrence of pMH1 and pM41 DNA sequences among various spiroplasmas. pM41 or a closely related plasmid was found in three other S. citri strains and also seems to be present as an 8-Kbp plasmid in three spiroplasmas not belonging to the S. citri species. Up to now, pMH1 had been found as a free 7-Kbp plasmid only in the S. citri strain MH. However, DNA sequences corresponding to the entire pMH1 DNA have been found to be integrated in the high-molecular-weight-DNA molecules and perhaps the chromosomal DNA itself of two other S. citri strains. DNA sequences hybridizable with pMH1 DNA have also been found to be integrated into high-molecular-weight-DNA molecules from several spiroplasma strains not belonging to the S. citri species.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><country name="France"><noRegion><name sortKey="Mouches, C" sort="Mouches, C" uniqKey="Mouches C" first="C." last="Mouches">C. Mouches</name></noRegion><name sortKey="Barroso, G" sort="Barroso, G" uniqKey="Barroso G" first="G." last="Barroso">G. Barroso</name><name sortKey="Bove, J M" sort="Bove, J M" uniqKey="Bove J" first="J. M." last="Bové">J. M. Bové</name><name sortKey="Gadeau, A" sort="Gadeau, A" uniqKey="Gadeau A" first="A." last="Gadeau">A. Gadeau</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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